Untangling the brain's neuroinflammatory and neurodegenerative transcriptional responses.
Ontology highlight
ABSTRACT:
SUBMITTER: Srinivasan K
PROVIDER: S-EPMC4844685 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Publications
Untangling the brain's neuroinflammatory and neurodegenerative transcriptional responses.
Srinivasan Karpagam K Friedman Brad A BA Larson Jessica L JL Lauffer Benjamin E BE Goldstein Leonard D LD Appling Laurie L LL Borneo Jovencio J Poon Chungkee C Ho Terence T Cai Fang F Steiner Pascal P van der Brug Marcel P MP Modrusan Zora Z Kaminker Joshua S JS Hansen David V DV
Nature communications 20160421
A common approach to understanding neurodegenerative disease is comparing gene expression in diseased versus healthy tissues. We illustrate that expression profiles derived from whole tissue RNA highly reflect the degenerating tissues' altered cellular composition, not necessarily transcriptional regulation. To accurately understand transcriptional changes that accompany neuropathology, we acutely purify neurons, astrocytes and microglia from single adult mouse brains and analyse their transcrip ...[more]
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