Mass spectrometry-based monitoring of millisecond protein-ligand binding dynamics using an automated microfluidic platform.
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ABSTRACT:
SUBMITTER: Cong Y
PROVIDER: S-EPMC4846533 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Publications
Mass spectrometry-based monitoring of millisecond protein-ligand binding dynamics using an automated microfluidic platform.
Cong Yongzheng Y Katipamula Shanta S Trader Cameron D CD Orton Daniel J DJ Geng Tao T Baker Erin S ES Kelly Ryan T RT
Lab on a chip 20160401 9
Characterizing protein-ligand binding dynamics is crucial for understanding protein function and for developing new therapeutic agents. We present a novel microfluidic platform that features rapid mixing of protein and ligand solutions, variable incubation times, and an integrated electrospray ionization source for mass spectrometry-based monitoring of protein-ligand binding dynamics. This platform offers many advantages, including solution-based binding, label-free detection, automated operatio ...[more]
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