Culture phases, cytotoxicity and protein expressions of agarose hydrogel induced Sp2/0, A549, MCF-7 cell line 3D cultures.
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ABSTRACT:
SUBMITTER: Ravi M
PROVIDER: S-EPMC4846635 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Publications
Culture phases, cytotoxicity and protein expressions of agarose hydrogel induced Sp2/0, A549, MCF-7 cell line 3D cultures.
Ravi Maddaly M Kaviya S R SR Paramesh V V
Cytotechnology 20141105 3
Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-induced cytotoxicity are studied. Also, the 3D aggregates of the three cell lines were reverted into 2D cultures and the protein profile differences among the 2D, 3D and revert cultur ...[more]
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