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Objective Morphological Quantification of Microscopic Images Using a Fast Fourier Transform (FFT) Analysis.


ABSTRACT: Quantification of immunohistochemistry (IHC) and immunofluorescence (IF) using image intensity depends on a number of variables. These variables add a subjective complexity in keeping a standard within and between laboratories. Fast Fourier Transformation (FFT) algorithms, however, allow for a rapid and objective quantification (via statistical analysis) using cell morphologies when the microscopic structures are oriented or aligned. Quantification of alignment is given in terms of a ratio of FFT intensity to the intensity of an orthogonal angle, giving a numerical value of the alignment of the microscopic structures. This allows for a more objective analysis than alternative approaches, which rely upon relative intensities.

SUBMITTER: Taylor SE 

PROVIDER: S-EPMC4849894 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Objective Morphological Quantification of Microscopic Images Using a Fast Fourier Transform (FFT) Analysis.

Taylor Samuel E SE   Cao Tuoxin T   Talauliker Pooja M PM   Lifshitz Jonathan J  

Current protocols essential laboratory techniques 20131023 Suppl 7


Quantification of immunohistochemistry (IHC) and immunofluorescence (IF) using image intensity depends on a number of variables. These variables add a subjective complexity in keeping a standard within and between laboratories. Fast Fourier Transformation (FFT) algorithms, however, allow for a rapid and objective quantification (via statistical analysis) using cell morphologies when the microscopic structures are oriented or aligned. Quantification of alignment is given in terms of a ratio of FF  ...[more]

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