Ontology highlight
ABSTRACT:
SUBMITTER: Taylor SE
PROVIDER: S-EPMC4849894 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Taylor Samuel E SE Cao Tuoxin T Talauliker Pooja M PM Lifshitz Jonathan J
Current protocols essential laboratory techniques 20131023 Suppl 7
Quantification of immunohistochemistry (IHC) and immunofluorescence (IF) using image intensity depends on a number of variables. These variables add a subjective complexity in keeping a standard within and between laboratories. Fast Fourier Transformation (FFT) algorithms, however, allow for a rapid and objective quantification (via statistical analysis) using cell morphologies when the microscopic structures are oriented or aligned. Quantification of alignment is given in terms of a ratio of FF ...[more]