Ontology highlight
ABSTRACT:
SUBMITTER: Ono M
PROVIDER: S-EPMC4851398 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Ono Motoharu M Yamada Kayo K Bensaddek Dalila D Afzal Vackar V Biddlestone John J Ortmann Brian B Mudie Sharon S Boivin Vincent V Scott Michelle S MS Rocha Sonia S Lamond Angus I AI
PloS one 20160429 4
The snoMEN (snoRNA Modulator of gene ExpressioN) vector technology was developed from a human box C/D snoRNA, HBII-180C, which contains an internal sequence that can be manipulated to make it complementary to RNA targets, allowing knock-down of targeted genes. Here we have screened additional human nucleolar snoRNAs and assessed their application for gene specific knock-downs to improve the efficiency of snoMEN vectors. We identify and characterise a new snoMEN vector, termed 47snoMEN, that is d ...[more]