A Zebrafish Genetic Screen Identifies Neuromedin U as a Regulator of Sleep/Wake States.

ABSTRACT: Neuromodulation of arousal states ensures that an animal appropriately responds to its environment and engages in behaviors necessary for survival. However, the molecular and circuit properties underlying neuromodulation of arousal states such as sleep and wakefulness remain unclear. To tackle this challenge in a systematic and unbiased manner, we performed a genetic overexpression screen to identify genes that affect larval zebrafish arousal. We found that the neuropeptide neuromedin U (Nmu) promotes hyperactivity and inhibits sleep in zebrafish larvae, whereas nmu mutant animals are hypoactive. We show that Nmu-induced arousal requires Nmu receptor 2 and signaling via corticotropin releasing hormone (Crh) receptor 1. In contrast to previously proposed models, we find that Nmu does not pr

SUBMITTER: Chiu CN 

PROVIDER: S-EPMC4851465 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature