Ontology highlight
ABSTRACT:
SUBMITTER: Schmidt FI
PROVIDER: S-EPMC4854733 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Schmidt Florian I FI Lu Alvin A Chen Jeff W JW Ruan Jianbin J Tang Catherine C Wu Hao H Ploegh Hidde L HL
The Journal of experimental medicine 20160411 5
Myeloid cells assemble inflammasomes in response to infection or cell damage; cytosolic sensors activate pro-caspase-1, indirectly for the most part, via the adaptors ASC and NLRC4. This leads to secretion of proinflammatory cytokines and pyroptosis. To explore complex formation under physiological conditions, we generated an alpaca single domain antibody, VHHASC, which specifically recognizes the CARD of human ASC via its type II interface. VHHASC not only impairs ASC(CARD) interactions in vitr ...[more]