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Gut macrophage phenotype is dependent on the tumor microenvironment in colorectal cancer.

ABSTRACT: In contrast to many cancers, a high infiltration of macrophages in colorectal cancer (CRC) has been associated with improved prognosis for patients. Cytokines and other stimuli from the tumor microenvironment affect monocyte to macrophage maturation and subsequent phenotype and function. Heterogeneous myeloid populations were identified using a novel flow cytometry panel in both tumor and paired non-tumor bowel (NTB) from CRC patients. The frequency of macrophage subsets with a gut-conditioned phenotype was lower in tumor compared with NTB. We used an in vitro system to show that two of the macrophage populations represented pro-inflammatory and anti-inflammatory phenotypes. Conditioned media that contained high levels of interleukin-6 promoted and maintained an anti-inflammatory phenotype in vitro. This study demonstrates the plasticity and heterogeneity of macrophage subtypes in human CRC, and the feasibility of studying complex populations. Ex vivo experiments demonstrate that macrophage subsets are influenced by the tumor microenvironment.

SUBMITTER: Norton SE

PROVIDER: S-EPMC4855270 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Gut macrophage phenotype is dependent on the tumor microenvironment in colorectal cancer.

Norton Samuel E SE Dunn Elliott T J ET McCall John L JL Munro Fran F Kemp Roslyn A RA

Clinical & translational immunology 20160429 4

In contrast to many cancers, a high infiltration of macrophages in colorectal cancer (CRC) has been associated with improved prognosis for patients. Cytokines and other stimuli from the tumor microenvironment affect monocyte to macrophage maturation and subsequent phenotype and function. Heterogeneous myeloid populations were identified using a novel flow cytometry panel in both tumor and paired non-tumor bowel (NTB) from CRC patients. The frequency of macrophage subsets with a gut-conditioned p ...[more]

PMID: 27195119

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Gut macrophage phenotype is dependent on the tumor microenvironment in colorectal cancer.

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Gut macrophage phenotype is dependent on the tumor microenvironment in colorectal cancer.

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