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A dynamic look backward and forward.

ABSTRACT: The 2015 Gunther Laukien Prize recognized solution NMR studies of protein dynamics and thermodynamics. This Perspective surveys aspects of the development and application of NMR spin relaxation for investigations of protein flexibility and function over multiple time scales in solution. Methods highlighted include analysis of overall rotational diffusion, theoretical descriptions of R1? relaxation, and molecular dynamics simulations to interpret NMR spin relaxation. Applications are illustrated for the zinc-finger domain Xfin-31, the calcium-binding proteins calbindin D9k and calmodulin, and the bZip transcription factor of GCN4.

SUBMITTER: Palmer AG

PROVIDER: S-EPMC4856014 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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A dynamic look backward and forward.

Palmer Arthur G AG

Journal of magnetic resonance (San Diego, Calif. : 1997) 20160211

The 2015 Gunther Laukien Prize recognized solution NMR studies of protein dynamics and thermodynamics. This Perspective surveys aspects of the development and application of NMR spin relaxation for investigations of protein flexibility and function over multiple time scales in solution. Methods highlighted include analysis of overall rotational diffusion, theoretical descriptions of R1ρ relaxation, and molecular dynamics simulations to interpret NMR spin relaxation. Applications are illustrated ...[more]

PMID: 26899226

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