Activity in varicosities within the myenteric plexus between and during the colonic migrating motor complex in the isolated murine large intestine.

ABSTRACT:

Background

  Neuronal communication within the myenteric plexus occurs when action potentials along nerve fibers produce Ca(2+) transients in varicosities leading to exocytosis of vesicles and neurotransmitters release. We used Ca(2+) transients in varicosities to monitor action potential activity in myenteric nerve pathways both between and during the colonic migrating motor complex (CMMC) in the isolated murine colon.

Methods

  Strips of longitudinal muscle were removed to reveal the myenteric ganglia, which were then loaded with Fluo-4.

Key results

  Many varicosities, including synaptotagmin 1 labeled varicosities, exhibited ongoing Ca(2+) transients (duration of unitary Ca(2+) transient 3.9 s). Between CMMCs, varicosities fired at a frequency of 0.6 Hz, which cor

SUBMITTER: Bayguinov PO 

PROVIDER: S-EPMC4856478 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature