Involvement of condensin-directed gene associations in the organization and regulation of chromosome territories during the cell cycle.

ABSTRACT: Chromosomes are not randomly disposed in the nucleus but instead occupy discrete sub-nuclear domains, referred to as chromosome territories. The molecular mechanisms that underlie the formation of chromosome territories and how they are regulated during the cell cycle remain largely unknown. Here, we have developed two different chromosome-painting approaches to address how chromosome territories are organized in the fission yeast model organism. We show that condensin frequently associates RNA polymerase III-transcribed genes (tRNA and 5S rRNA) that are present on the same chromosomes, and that the disruption of these associations by condensin mutations significantly compromises the chromosome territory arrangement. We also find that condensin-dependent intra-chromosomal gene associations

SUBMITTER: Iwasaki O 

PROVIDER: S-EPMC4856965 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature