?-3 Tear Film Lipids Correlate With Clinical Measures of Dry Eye.

ABSTRACT: PURPOSE:?-3 and ?-6 polyunsaturated fatty acids modulate inflammatory processes throughout the body through distinct classes of lipid mediators that possess both proinflammatory and proresolving properties. The purpose of this cross-sectional study was to explore the relationship between lipid profiles in human tears and dry eye (DE) symptoms and signs. METHODS:Forty-one patients with normal eyelid and corneal anatomy were prospectively recruited from a Veterans Administration Hospital over 18 months. Symptoms and signs of DE were assessed, and tear samples was analyzed by mass spectrometry-based lipidomics. Statistical analyses comparing the relationship between tear film lipids and DE included Pearson/Spearman correlations and t-tests. RESULTS:Arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) were present in more than 90% of tear film samples. The ratio of ?-6 (AA) to ?-3 (DHA+EPA) fatty acids was correlated with multiple measures of tear film dysfunction (tear breakup time, Schirmer 2 scores, and corneal staining; all P < 0.05). Arachidonic acid-derived prostaglandin E2 was detected in the majority of samples and correlated with low tear osmolarity, meibomian gland plugging, and corneal staining. CONCLUSIONS:Both ?-3 and ?-6 lipid circuits are activated in the human tear film. The ratio of ?-6:?-3 tear lipids is elevated in DE patients in proportion to the degree of tear film dysfunction and corneal staining. Metabolic deficiency of ?-3 tear film lipids may be a driver of chronic ocular surface inflammation in DE.

SUBMITTER: Walter SD 

PROVIDER: S-EPMC4857833 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature