Ontology highlight
ABSTRACT:
SUBMITTER: Chiang YL
PROVIDER: S-EPMC4860349 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Chiang Ying-Ling YL Lin Hening H
Organic & biomolecular chemistry 20160121 7
Sirtuins are NAD-dependent lysine deacylases that play critical roles in cellular regulation and are implicated in human diseases. Modulators of sirtuins are needed as tools for investigating their biological functions and possible therapeutic applications. However, the discovery of sirtuin modulators is hampered by the lack of efficient sirtuin assays. Here we report an improved fluorogenic assay for SIRT1, SIRT2, and SIRT3 using a new substrate, a myristoyl peptide with a C-terminal aminocouma ...[more]