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Rapid assessment of iron in blood plasma and serum by spectrophotometry with cloud-point extraction.


ABSTRACT: Rapid photometric assessment of iron in blood plasma and serum by a simple procedure after the extraction of iron(II) complex with 1-nitroso-2-naphthol in the micellar phase of a nonionic surfactant at the cloud point upon heating (pH range is 4.5-6.3) is proposed. The procedure trueness was verified using a standard reference protocol using bathophenanthroline. The advantages of the procedure are higher sensitivity than the reference protocol: the limit of detection is 0.03 ?g/mL, the limit of quantitation is 0.1 ?g/mL, the determination range is 0.1 - 2.8 ?g/mL (RSD 0.02-0.10). Copper does not interfere with the iron assessment.

SUBMITTER: Samarina T 

PROVIDER: S-EPMC4863669 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Rapid assessment of iron in blood plasma and serum by spectrophotometry with cloud-point extraction.

Samarina Tatyana T   Proskurnin Mikhail M  

F1000Research 20150825


Rapid photometric assessment of iron in blood plasma and serum by a simple procedure after the extraction of iron(II) complex with 1-nitroso-2-naphthol in the micellar phase of a nonionic surfactant at the cloud point upon heating (pH range is 4.5-6.3) is proposed. The procedure trueness was verified using a standard reference protocol using bathophenanthroline. The advantages of the procedure are higher sensitivity than the reference protocol: the limit of detection is 0.03 μg/mL, the limit of  ...[more]

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