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Monocyte enhancers are highly altered in systemic lupus erythematosus.

ABSTRACT:

Objective

Histone modifications set transcriptional competency and can perpetuate pathologic expression patterns. We defined systemic lupus erythematosus (SLE)-specific changes in H3K4me3 and K3K27me3, histone marks of gene activation and repression, respectively.

Methods

We used ChIP-seq to define histone modifications in monocytes from SLE patients and controls.

Results

Both promoters and enhancers exhibited significant changes in histone methylation in SLE. Regions with differential H3K4me3 in SLE were significantly enriched in potential interferon-related transcription factor binding sites and pioneer transcription factor sites.

Conclusion

Enhancer activation defines the character of the cell and our data support extensive disease effects in monocytes, a particularly plastic lineage. Type I interferons not only drive altered gene expression but may also alter the character of the cell through chromatin modifications.

SUBMITTER: Shi L

PROVIDER: S-EPMC4864065 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Monocyte enhancers are highly altered in systemic lupus erythematosus.

Shi Lihua L Zhang Zhe Z Song Li L Leung Yiu Tak YT Petri Michelle A MA Sullivan Kathleen E KE

Epigenomics 20151007 6

<h4>Objective</h4>Histone modifications set transcriptional competency and can perpetuate pathologic expression patterns. We defined systemic lupus erythematosus (SLE)-specific changes in H3K4me3 and K3K27me3, histone marks of gene activation and repression, respectively.<h4>Methods</h4>We used ChIP-seq to define histone modifications in monocytes from SLE patients and controls.<h4>Results</h4>Both promoters and enhancers exhibited significant changes in histone methylation in SLE. Regions with ...[more]

PMID: 26442457

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