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Optimising selective excitation pulses to maximise saturation transfer difference NMR spectroscopy.

ABSTRACT: A simple method is presented that optimizes the STD NMR Gaussian pulse to deliver significant increases in STD amplification factors with minimal perturbation of the ligand. This approach is practically demonstrated using the wheat-germ agglutinin/N-acetyl-D-glucosamine protein-ligand system.

SUBMITTER: Ley NB 

PROVIDER: S-EPMC4864509 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Optimising selective excitation pulses to maximise saturation transfer difference NMR spectroscopy.

Ley Nathan B NB   Rowe Michelle L ML   Williamson Richard A RA   Howard Mark J MJ  

RSC advances 20140108 14

A simple method is presented that optimizes the STD NMR Gaussian pulse to deliver significant increases in STD amplification factors with minimal perturbation of the ligand. This approach is practically demonstrated using the wheat-germ agglutinin/N-acetyl-D-glucosamine protein-ligand system. ...[more]

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