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MiR-145a-5p Promotes Myoblast Differentiation.

ABSTRACT: MicroRNAs are a class of 18-22-nucleotide noncoding RNAs that posttranscriptionally regulate gene expression and have been shown to play an important role during myoblast differentiation. In this study, we found that the expression of miR-145a-5p was gradually increased during C2C12 myoblast differentiation, and miR-145a-5p inhibitors or mimics significantly suppressed or promoted the relative expression of specific myogenesis related marker genes. Moreover, overexpression or inhibition of miR-145a-5p enhanced or repressed the expression of some special genes involved in the endogenous Wnt signaling pathway during C2C12 myoblast differentiation, including Wnt5a, LRP5, Axin2, and ?-catenin. These results indicated that miR-145a-5p might be considered as a new myogenic differentiation-associated microRNA that can promote C2C12 myoblast differentiation by enhancing genes related to myoblasts differentiation.

SUBMITTER: Du J

PROVIDER: S-EPMC4864570 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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miR-145a-5p Promotes Myoblast Differentiation.

Du Jingjing J Li Qiang Q Shen Linyuan L Lei Huaigang H Luo Jia J Liu Yihui Y Zhang Peiwen P Pu Qiang Q Zhang Yi Y Shuai Surong S Li Xuewei X Zhang Shunhua S Zhu Li L

BioMed research international 20160428

MicroRNAs are a class of 18-22-nucleotide noncoding RNAs that posttranscriptionally regulate gene expression and have been shown to play an important role during myoblast differentiation. In this study, we found that the expression of miR-145a-5p was gradually increased during C2C12 myoblast differentiation, and miR-145a-5p inhibitors or mimics significantly suppressed or promoted the relative expression of specific myogenesis related marker genes. Moreover, overexpression or inhibition of miR-1 ...[more]

PMID: 27239472

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