Dataset Information

Improved specificity of hippocampal memory trace labeling.

ABSTRACT: Recent studies have focused on the identification and manipulation of memory traces in rodent models. The two main mouse models utilized are either a CreER(T2) /loxP tamoxifen (TAM)- or a tetracycline transactivator/tetracycline-response element doxycycline-inducible system. These systems, however, could be improved to label a more specific population of activated neurons corresponding to behavior. Here, we sought to identify an improved selective estrogen receptor (ER) modulator (SERM) in which we could label an individual memory trace in ArcCreER(T2) mice. We found that 4-hydroxytamoxifen (4-OHT) is a selective SERM in the ArcCreER(T2) × Rosa26-CAG-stop(flox) -channelrhodospin (ChR2)-enhanced yellow fluorescent protein (eYFP) mice. The half-life of 4-OHT is shorter than TAM, allowing for more specificity of memory trace labeling. Furthermore, 4-OHT allowed for context-specific labeling in the dentate gyrus and CA3. In summary, we believe that 4-OHT improves the specificity of memory trace labeling and will allow for refined memory trace studies in the future. © 2015 Wiley Periodicals, Inc.

SUBMITTER: Cazzulino AS

PROVIDER: S-EPMC4867142 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Improved specificity of hippocampal memory trace labeling.

Cazzulino Alejandro S AS Martinez Randy R Tomm Nicole K NK Denny Christine A CA

Hippocampus 20160120 6

Recent studies have focused on the identification and manipulation of memory traces in rodent models. The two main mouse models utilized are either a CreER(T2) /loxP tamoxifen (TAM)- or a tetracycline transactivator/tetracycline-response element doxycycline-inducible system. These systems, however, could be improved to label a more specific population of activated neurons corresponding to behavior. Here, we sought to identify an improved selective estrogen receptor (ER) modulator (SERM) in which ...[more]

PMID: 26662713

Similar Datasets

Project description:The hippocampus plays important roles in memory formation and retrieval through sharp-wave-ripples. Recent studies have shown that certain neuron populations in the prefrontal cortex (PFC) exhibit coordinated reactivations during awake ripple events. These experimental findings suggest that the awake ripple is an important biomarker, through which the hippocampus interacts with the neocortex to assist memory formation and retrieval. However, the computational mechanisms of this ripple based hippocampal-cortical coordination are still not clear due to the lack of unified models that include both the hippocampal and cortical networks. In this work, using a coupled biophysical model of both CA1 and PFC, we investigate possible mechanisms of hippocampal-cortical memory trace transfer and the conditions that assist reactivation of the transferred memory traces in the PFC. To validate our model, we first show that the local field potentials generated in the hippocampus and PFC exhibit ripple range activities that are consistent with the recent experimental studies. Then we demonstrate that during ripples, sequence replays can successfully transfer the information stored in the hippocampus to the PFC recurrent networks. We investigate possible mechanisms of memory retrieval in PFC networks. Our results suggest that the stored memory traces in the PFC network can be retrieved through two different mechanisms, namely the cell-specific input representing external stimuli and non-specific spontaneous background noise representing spontaneous memory recall events. Importantly, in both cases, the memory reactivation quality is robust to network connection loss. Finally, we find out that the quality of sequence reactivations is enhanced by both increased number of SWRs and an optimal background noise intensity, which tunes the excitability of neurons to a proper level. Our study presents a mechanistic explanation for the memory trace transfer from the hippocampus to neocortex through ripple coupling in awake states and reports two different mechanisms by which the stored memory traces can be successfully retrieved.

Dataset Information

Improved specificity of hippocampal memory trace labeling.

Publications

Improved specificity of hippocampal memory trace labeling.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets