Unknown

Dataset Information

0

Polycomb repressive complex 1 controls uterine decidualization.


ABSTRACT: Uterine stromal cell decidualization is an essential part of the reproductive process. Decidual tissue development requires a highly regulated control of the extracellular tissue remodeling; however the mechanism of this regulation remains unknown. Through systematic expression studies, we detected that Cbx4/2, Rybp, and Ring1B [components of polycomb repressive complex 1 (PRC1)] are predominantly utilized in antimesometrial decidualization with polyploidy. Immunofluorescence analyses revealed that PRC1 members are co-localized with its functional histone modifier H2AK119ub1 (mono ubiquitination of histone-H2A at lysine-119) in polyploid cell. A potent small-molecule inhibitor of Ring1A/B E3-ubiquitin ligase or siRNA-mediated suppression of Cbx4 caused inhibition of H2AK119ub1, in conjunction with perturbation of decidualization and polyploidy development, suggesting a role for Cbx4/Ring1B-containing PRC1 in these processes. Analyses of genetic signatures by RNA-seq studies showed that the inhibition of PRC1 function affects 238 genes (154 up and 84 down) during decidualization. Functional enrichment analyses identified that about 38% genes primarily involved in extracellular processes are specifically targeted by PRC1. Furthermore, ~15% of upregulated genes exhibited a significant overlap with the upregulated Bmp2 null-induced genes in mice. Overall, Cbx4/Ring1B-containing PRC1 controls decidualization via regulation of extracellular gene remodeling functions and sheds new insights into underlying molecular mechanism(s) through transcriptional repression regulation.

SUBMITTER: Bian F 

PROVIDER: S-EPMC4867636 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Polycomb repressive complex 1 controls uterine decidualization.

Bian Fenghua F   Gao Fei F   Kartashov Andrey V AV   Jegga Anil G AG   Barski Artem A   Das Sanjoy K SK  

Scientific reports 20160516


Uterine stromal cell decidualization is an essential part of the reproductive process. Decidual tissue development requires a highly regulated control of the extracellular tissue remodeling; however the mechanism of this regulation remains unknown. Through systematic expression studies, we detected that Cbx4/2, Rybp, and Ring1B [components of polycomb repressive complex 1 (PRC1)] are predominantly utilized in antimesometrial decidualization with polyploidy. Immunofluorescence analyses revealed t  ...[more]

Similar Datasets

2017-04-18 | GSE80623 | GEO
| S-EPMC3053347 | biostudies-literature
| S-EPMC2807366 | biostudies-literature
| S-EPMC6921245 | biostudies-literature
| S-EPMC4951031 | biostudies-literature
| S-EPMC3823624 | biostudies-literature
| S-EPMC3482686 | biostudies-literature
| S-EPMC6635771 | biostudies-literature
| S-EPMC5555192 | biostudies-literature
| S-EPMC5540264 | biostudies-literature