Ontology highlight
ABSTRACT:
SUBMITTER: Thomsen ER
PROVIDER: S-EPMC4869711 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Thomsen Elliot R ER Mich John K JK Yao Zizhen Z Hodge Rebecca D RD Doyle Adele M AM Jang Sumin S Shehata Soraya I SI Nelson Angelique M AM Shapovalova Nadiya V NV Levi Boaz P BP Ramanathan Sharad S
Nature methods 20151116 1
The diverse progenitors that give rise to the human neocortex have been difficult to characterize because progenitors, particularly radial glia (RG), are rare and are defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed Fixed and Recovered Intact Single-cell RNA (FRISCR), a method for profiling the transcriptomes of individual fixed, stained and sorted cells. Using FRISCR, we profiled primary human RG that constitute only 1% of th ...[more]