Dataset Information

Gene signature of the post-Chernobyl papillary thyroid cancer.

ABSTRACT:

Purpose

Following the nuclear accidents in Chernobyl and later in Fukushima, the nuclear community has been faced with important issues concerning how to search for and diagnose biological consequences of low-dose internal radiation contamination. Although after the Chernobyl accident an increase in childhood papillary thyroid cancer (PTC) was observed, it is still not clear whether the molecular biology of PTCs associated with low-dose radiation exposure differs from that of sporadic PTC.

Methods

We investigated tissue samples from 65 children/young adults with PTC using DNA microarray (Affymetrix, Human Genome U133 2.0 Plus) with the aim of identifying molecular differences between radiation-induced (exposed to Chernobyl radiation, ECR) and sporadic PTC. All participants were resident in the same region so that confounding factors related to genetics or environment were minimized.

Results

There were small but significant differences in the gene expression profiles between ECR and non-ECR PTC (global test, p?ConclusionSignificant, but subtle, differences in gene expression in the post-Chernobyl PTC are associated with previous low-dose radiation exposure.

SUBMITTER: Handkiewicz-Junak D

PROVIDER: S-EPMC4869750 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Publications

Gene signature of the post-Chernobyl papillary thyroid cancer.

Handkiewicz-Junak Daria D Swierniak Michal M Rusinek Dagmara D Oczko-Wojciechowska Małgorzata M Dom Genevieve G Maenhaut Carine C Unger Kristian K Detours Vincent V Bogdanova Tetiana T Thomas Geraldine G Likhtarov Ilya I Jaksik Roman R Kowalska Malgorzata M Chmielik Ewa E Jarzab Michal M Swierniak Andrzej A Jarzab Barbara B

European journal of nuclear medicine and molecular imaging 20160126 7

<h4>Purpose</h4>Following the nuclear accidents in Chernobyl and later in Fukushima, the nuclear community has been faced with important issues concerning how to search for and diagnose biological consequences of low-dose internal radiation contamination. Although after the Chernobyl accident an increase in childhood papillary thyroid cancer (PTC) was observed, it is still not clear whether the molecular biology of PTCs associated with low-dose radiation exposure differs from that of sporadic PT ...[more]

PMID: 26810418

Similar Datasets

Project description:Background:Exposure to ionizing radiation during childhood is a well-established risk factor for thyroid cancer. However, the genetic mechanisms of radiation-associated carcinogenesis remain not fully understood. Methods:In this study, we used targeted next-generation sequencing and RNA-Seq to study 65 papillary thyroid cancers (PTCs) from patients in the Ukrainian-American cohort with measurement-based iodine-131 (I-131) thyroid doses received as a result of the Chernobyl accident. We fitted linear regression models to evaluate differences in distribution of risk factors for PTC according to type of genetic alteration and logistic regression models to evaluate the I-131 dose response. All statistical tests were two-sided. Results:Driver mutations were identified in 96.9% of these thyroid cancers, including point mutations in 26.2% and gene fusions in 70.8% of cases. Novel driver fusions such as POR-BRAF, as well as STRN-ALK fusions that have not been implicated in radiation-associated cancer before, were found. The mean I-131 dose in cases with point mutations was 0.2 Gy (range = 0.013-1.05 Gy), statistically significantly lower than 1.4 Gy (range = 0.009-6.15 Gy) for cases with fusions (P < .001). No driver point mutations were found in tumors from individuals who received more than 1.1 Gy of radiation. Relative to tumors with point mutations, the proportion of tumors with gene fusions increased with radiation dose, reaching 87.8% among individuals exposed to 0.3 Gy or higher. With a limited study sample size, the estimated odds ratio at 1 Gy was 20.01 (95% confidence interval = 2.57 to 653.02, P < .001). In addition, after controlling for I-131 dose, we found higher odds ratios for gene fusion-positive PTCs associated with several specific demographic and geographic features. Conclusions:Our data provide support for a link between I-131 thyroid dose and generation of carcinogenic gene fusions, the predominant mechanism of thyroid cancer associated with radiation exposure from the Chernobyl accident.

Dataset Information

Gene signature of the post-Chernobyl papillary thyroid cancer.

Purpose

Methods

Results

Publications

Gene signature of the post-Chernobyl papillary thyroid cancer.

Similar Datasets

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