Targeting membrane proteins for antibody discovery using phage display.
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ABSTRACT:
SUBMITTER: Jones ML
PROVIDER: S-EPMC4870581 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Publications
Targeting membrane proteins for antibody discovery using phage display.
Jones Martina L ML Alfaleh Mohamed A MA Kumble Sumukh S Zhang Shuo S Osborne Geoffrey W GW Yeh Michael M Arora Neetika N Hou Jeff Jia Cheng JJ Howard Christopher B CB Chin David Y DY Mahler Stephen M SM
Scientific reports 20160518
A critical factor in the successful isolation of new antibodies by phage display is the presentation of a correctly folded antigen. While this is relatively simple for soluble proteins which can be purified and immobilized onto a plastic surface, membrane proteins offer significant challenges for antibody discovery. Whole cell panning allows presentation of the membrane protein in its native conformation, but is complicated by a low target antigen density, high background of irrelevant antigens ...[more]
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