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Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets.

ABSTRACT: We demonstrate a simple and efficient method for producing ultrathin Bessel ('non-diffracting') light sheets of any color using a line-shaped beam and an annulus filter. With this robust and cost-effective technology, we obtained two-color, 3D images of biological samples with lateral/axial resolution of 250?nm/400?nm, and high-speed, 4D volume imaging of 20??m sized live sample at 1?Hz temporal resolution.

SUBMITTER: Zhao T

PROVIDER: S-EPMC4870613 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets.

Zhao Teng T Lau Sze Cheung SC Wang Ying Y Su Yumian Y Wang Hao H Cheng Aifang A Herrup Karl K Ip Nancy Y NY Du Shengwang S Loy M M T MM

Scientific reports 20160518

We demonstrate a simple and efficient method for producing ultrathin Bessel ('non-diffracting') light sheets of any color using a line-shaped beam and an annulus filter. With this robust and cost-effective technology, we obtained two-color, 3D images of biological samples with lateral/axial resolution of 250 nm/400 nm, and high-speed, 4D volume imaging of 20 μm sized live sample at 1 Hz temporal resolution. ...[more]

PMID: 27189786

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