Unknown

Dataset Information

0

Target-Specific Glycinergic Transmission from VGluT3-Expressing Amacrine Cells Shapes Suppressive Contrast Responses in the Retina.


ABSTRACT: Neurons that release more than one transmitter exist throughout the CNS. Yet, how these neurons deploy multiple transmitters and shape the function of specific circuits is not well understood. VGluT3-expressing amacrine cells (VG3-ACs) provide glutamatergic input to ganglion cells activated by contrast and motion. Using optogenetics, we find that VG3-ACs provide selective glycinergic input to a retinal ganglion cell type suppressed by contrast and motion (SbC-RGCs). Firing of SbC-RGCs is suppressed at light ON and OFF over a broad range of stimulus sizes. Anatomical circuit reconstructions reveal that VG3-ACs form inhibitory synapses preferentially on processes that link ON and OFF arbors of SbC-RGC dendrites. Removal of VG3-ACs from mature circuits reduces inhibition and attenuates spike suppression of SbC-RGCs in a contrast- and size-selective manner, illustrating the modularity of retinal circuits. VG3-ACs thus use dual transmitters in a target-specific manner and shape suppressive contrast responses in the retina by glycinergic transmission.

SUBMITTER: Tien NW 

PROVIDER: S-EPMC4871735 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Target-Specific Glycinergic Transmission from VGluT3-Expressing Amacrine Cells Shapes Suppressive Contrast Responses in the Retina.

Tien Nai-Wen NW   Kim Tahnbee T   Kerschensteiner Daniel D  

Cell reports 20160505 7


Neurons that release more than one transmitter exist throughout the CNS. Yet, how these neurons deploy multiple transmitters and shape the function of specific circuits is not well understood. VGluT3-expressing amacrine cells (VG3-ACs) provide glutamatergic input to ganglion cells activated by contrast and motion. Using optogenetics, we find that VG3-ACs provide selective glycinergic input to a retinal ganglion cell type suppressed by contrast and motion (SbC-RGCs). Firing of SbC-RGCs is suppres  ...[more]

Similar Datasets

| S-EPMC8741526 | biostudies-literature
| S-EPMC4824647 | biostudies-literature
| S-EPMC10997783 | biostudies-literature
| S-EPMC4254642 | biostudies-literature
| S-EPMC5653236 | biostudies-literature
| S-EPMC4150031 | biostudies-literature
| S-EPMC5614429 | biostudies-literature
| S-EPMC5580440 | biostudies-literature
| S-EPMC2575372 | biostudies-literature
| S-EPMC2664096 | biostudies-literature