Ontology highlight
ABSTRACT:
SUBMITTER: Chu CC
PROVIDER: S-EPMC4872867 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Organic & biomolecular chemistry 20160501 20
We evaluate the ability of hexahistidine (His6) tags on peptide and protein substrates to recruit deoxyribozymes for modifying those substrates. For two different deoxyribozymes, one that creates tyrosine-RNA nucleopeptides and another that phosphorylates tyrosine side chains, we find substantial improvements in yield, kobs, and Km for peptide substrates due to recruiting by His6/Cu(2+). However, the recruiting benefits of the histidine tag are not observed for larger protein substrates, likely ...[more]