Dataset Information

Additive Expression of Consolidated Memory through Drosophila Mushroom Body Subsets.

ABSTRACT: Associative olfactory memory in Drosophila has two components called labile anesthesia-sensitive memory and consolidated anesthesia-resistant memory (ARM). Mushroom body (MB) is a brain region critical for the olfactory memory and comprised of 2000 neurons that can be classified into ??, ?'?', and ? neurons. Previously we demonstrated that two parallel pathways mediated ARM consolidation: the serotonergic dorsal paired medial (DPM)-?? neurons and the octopaminergic anterior paired lateral (APL)-?'?' neurons. This finding prompted us to ask how this composite ARM is retrieved. Here, we showed that blocking the output of ?? neurons and that of ?'?' neurons each impaired ARM retrieval, and blocking both simultaneously had an additive effect. Knockdown of radish and oct?2R in ?? and ?'?' neurons, respectively, impaired ARM. A combinatorial assay of radish mutant background rsh1 and neurotransmission blockade confirmed that ARM retrieved from ?'?' neuron output is independent of radish. We identified MBON-?2?'2a and MBON-?'2mp as the MB output neurons downstream of ?? and ?'?' neurons, respectively, whose glutamatergic transmissions also additively contribute to ARM retrieval. Finally, we showed that ?'?' neurons could be functionally subdivided into ?'?'m neurons required for ARM retrieval, and ?'?'ap neurons required for ARM consolidation. Our work demonstrated that two parallel neural pathways mediating ARM consolidation in Drosophila MB additively contribute to ARM expression during retrieval.

SUBMITTER: Yang CH

PROVIDER: S-EPMC4873240 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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Additive Expression of Consolidated Memory through Drosophila Mushroom Body Subsets.

Yang Chu-Huai CH Shih Meng-Fu Maxwell MF Chang Ching-Ching CC Chiang Meng-Hsuan MH Shih Hsiang-Wen HW Tsai Ya-Lun YL Chiang Ann-Shyn AS Fu Tsai-Feng TF Wu Chia-Lin CL

PLoS genetics 20160519 5

Associative olfactory memory in Drosophila has two components called labile anesthesia-sensitive memory and consolidated anesthesia-resistant memory (ARM). Mushroom body (MB) is a brain region critical for the olfactory memory and comprised of 2000 neurons that can be classified into αβ, α'β', and γ neurons. Previously we demonstrated that two parallel pathways mediated ARM consolidation: the serotonergic dorsal paired medial (DPM)-αβ neurons and the octopaminergic anterior paired lateral (APL)- ...[more]

PMID: 27195782

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Project description:An animal's ability to learn and to form memories is essential for its survival. The fruit fly has proven to be a valuable model system for studies of learning and memory. One learned behavior in fruit flies is courtship conditioning. In Drosophila courtship conditioning, male flies learn not to court females during training with an unreceptive female. He retains a memory of this training and for several hours decreases courtship when subsequently paired with any female. Courtship conditioning is a unique learning paradigm; it uses a positive-valence stimulus, a female fly, to teach a male to decrease an innate behavior, courtship of the female. As such, courtship conditioning is not clearly categorized as either appetitive or aversive conditioning. The mushroom body (MB) region in the fruit fly brain is important for several types of memory; however, the precise subsets of intrinsic and extrinsic MB neurons necessary for courtship conditioning are unknown. Here, we disrupted synaptic signaling by driving a shibirets effector in precise subsets of MB neurons, defined by a collection of split-GAL4 drivers. Out of 75 lines tested, 32 showed defects in courtship conditioning memory. Surprisingly, we did not have any hits in the ? lobe Kenyon cells, a region previously implicated in courtship conditioning memory. We did find that several ? lobe extrinsic neurons were necessary for courtship conditioning memory. Overall, our memory hits in the dopaminergic neurons (DANs) and the mushroom body output neurons were more consistent with results from appetitive memory assays than aversive memory assays. For example, protocerebral anterior medial DANs were necessary for courtship memory, similar to appetitive memory, while protocerebral posterior lateral 1 (PPL1) DANs, important for aversive memory, were not needed. Overall, our results indicate that the MB circuits necessary for courtship conditioning memory coincide with circuits necessary for appetitive memory.

Dataset Information

Additive Expression of Consolidated Memory through Drosophila Mushroom Body Subsets.

Publications

Additive Expression of Consolidated Memory through Drosophila Mushroom Body Subsets.

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