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Preparation, Characterization, and Oxygenase Activity of a Photocatalytic Artificial Enzyme.


ABSTRACT: A bicyclo[6,1,0]nonyne-substituted 9-mesityl-10-methyl-acridinium cofactor was prepared and covalently linked to a prolyl oligopeptidase scaffold containing a genetically encoded 4-azido-L-phenylalanine residue in its active site. The resulting artificial enzyme catalyzed sulfoxidation when irradiated with visible light in the presence of air. This reaction proceeds by initial electron abstraction from the sulfide within the enzyme active site, and the protein scaffold extended the fluorescence lifetime of the acridium cofactor. The mode of sulfide activation and placement of the acridinium cofactor (5) in POP-ZA4 -5 make this artificial enzyme a promising platform for developing selective photocatalytic transformations.

SUBMITTER: Gu Y 

PROVIDER: S-EPMC4873458 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

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Preparation, Characterization, and Oxygenase Activity of a Photocatalytic Artificial Enzyme.

Gu Yifan Y   Ellis-Guardiola Ken K   Srivastava Poonam P   Lewis Jared C JC  

Chembiochem : a European journal of chemical biology 20150714 13


A bicyclo[6,1,0]nonyne-substituted 9-mesityl-10-methyl-acridinium cofactor was prepared and covalently linked to a prolyl oligopeptidase scaffold containing a genetically encoded 4-azido-L-phenylalanine residue in its active site. The resulting artificial enzyme catalyzed sulfoxidation when irradiated with visible light in the presence of air. This reaction proceeds by initial electron abstraction from the sulfide within the enzyme active site, and the protein scaffold extended the fluorescence  ...[more]

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