A novel automatable enzyme-coupled colorimetric assay for endo-1,4-?-glucanase (cellulase).
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ABSTRACT:
SUBMITTER: Mangan D
PROVIDER: S-EPMC4873538 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
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A novel automatable enzyme-coupled colorimetric assay for endo-1,4-β-glucanase (cellulase).
Mangan David D Cornaggia Claudio C McKie Vincent V Kargelis Tadas T McCleary Barry V BV
Analytical and bioanalytical chemistry 20160406 15
endo-1,4-β-Glucanase (endo-cellulase, EC 3.2.1.4) is one of the most widely used enzymes in industry. Despite its importance, improved methods for the rapid, selective, quantitative assay of this enzyme have been slow to emerge. In 2014, a novel enzyme-coupled assay that addressed many of the limitations of the existing assay methodology was reported. This involved the use of a bifunctional substrate chemically derived from cellotriose. Reported herein is a much improved version of this assay em ...[more]
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