Unknown

Dataset Information

0

Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis.


ABSTRACT: Colony-stimulating factor 1 receptor (CSF-1R) is an important regulator of monocytes/macrophages (MO/M?). Although several CSF-1R genes have been identified in teleosts, the precise role of CSF- 1R in ayu (Plecoglossus altivelis) remains unclear. In this study, we characterized the CSF-1R homologue from P. altivelis, and named it PaCSF-1R. Multiple sequence alignment and phylogenetic tree analysis showed that PaCSF-1R was most closely related to that of Japanese ricefish (Oryzias latipes). Tissue distribution and expression analysis showed that the PaCSF-1R transcript was mainly expressed in the head kidney-derived MO/M?, spleen, and head kidney, and its expression was significantly altered in various tissues upon Vibrio anguillarum infection. After PaCSF-1R neutralization for 48 h, the phagocytic activity of MO/M? was significantly decreased, suggesting that PaCSF-1R plays a role in regulating the phagocytic function of ayu MO/M?.

SUBMITTER: Chen Q 

PROVIDER: S-EPMC4876830 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis.

Chen Qiang Q   Lu Xin-Jiang XJ   Li Ming-Yun MY   Chen Jiong J  

Dong wu xue yan jiu = Zoological research 20160301 2


Colony-stimulating factor 1 receptor (CSF-1R) is an important regulator of monocytes/macrophages (MO/MΦ). Although several CSF-1R genes have been identified in teleosts, the precise role of CSF- 1R in ayu (Plecoglossus altivelis) remains unclear. In this study, we characterized the CSF-1R homologue from P. altivelis, and named it PaCSF-1R. Multiple sequence alignment and phylogenetic tree analysis showed that PaCSF-1R was most closely related to that of Japanese ricefish (Oryzias latipes). Tissu  ...[more]

Similar Datasets

| PRJDB3273 | ENA
| PRJDB6940 | ENA
| PRJDB13368 | ENA
| PRJNA513406 | ENA
| PRJDB4771 | ENA
| PRJNA80185 | ENA
| PRJNA78033 | ENA
| PRJNA509952 | ENA
| PRJNA296594 | ENA
| PRJNA257001 | ENA