Dataset Information

Apolipoprotein E Gene Polymorphisms in Saudi Patients with Systemic Lupus Erythematosus.

ABSTRACT: Apolipoprotein E (APOE) is a glycosylated protein with multiple biological properties. APOE gene polymorphism plays a central role in lipid metabolism and has recently been suggested to regulate inflammation. Our objective is to evaluate whether APOE polymorphism affects susceptibility to SLE. APOE genotyping was performed using ApoE StripAssay™ kit. Results indicated significantly higher frequencies of allele ?4 and genotype ?3/?4 and lower frequencies of allele ?3 and genotype ?3/?3 in SLE patients than controls. APOE ?2 allele was found in three patients, whereas it was absent in controls. The frequencies of allele ?4 and genotype ?3/?4 were significantly higher in SLE patients with renal involvement and those of alleles ?2, ?4 and genotypes ?2/?3, ?3/?4 were higher in patients with neuropsychiatric symptoms. It is concluded that APOE allele ?4 is associated with susceptibility risk/clinical manifestations of SLE and ?2 may increase its severity while ?3 is protective for SLE in Saudis.

SUBMITTER: Al-Rayes H

PROVIDER: S-EPMC4877081 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Apolipoprotein E Gene Polymorphisms in Saudi Patients with Systemic Lupus Erythematosus.

Al-Rayes Hannan H Huraib Ghaleb G Julkhuf Saeed S Arfin Misbahul M Tariq Mohammad M Al-Asmari Abdulrahman A

Clinical medicine insights. Arthritis and musculoskeletal disorders 20160522

Apolipoprotein E (APOE) is a glycosylated protein with multiple biological properties. APOE gene polymorphism plays a central role in lipid metabolism and has recently been suggested to regulate inflammation. Our objective is to evaluate whether APOE polymorphism affects susceptibility to SLE. APOE genotyping was performed using ApoE StripAssay™ kit. Results indicated significantly higher frequencies of allele ε4 and genotype ε3/ε4 and lower frequencies of allele ε3 and genotype ε3/ε3 in SLE pat ...[more]

PMID: 27257397

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Project description:Correlation between soluble vascular endothelial growth factor receptor-1 (sVEGFR-1) concentration, VEGFR1 gene polymorphisms and systemic lupus erythematosus (SLE) risk remains unclear. The present case-control study comprised 254 SLE patients, 385 other rheumatic diseases patients and 390 healthy controls. Serum levels of sVEGFR-1 were detected by enzyme-linked immunosorbent assay. Seven VEGFR1 genetic variants (rs2296188, rs9943922, rs2296283, rs7324510, rs9554322, rs9582036, rs9554320) were genotyped by KASP. Serum levels of sVEGFR-1 were up-regulated in SLE and positively correlated with disease activity. Furthermore, serum sVEGFR-1 presented a distinctive elevation in SLE in comparison with other rheumatic diseases. Frequencies of allele T of rs2296283 and allele G of rs9554322 were significant lower in SLE patients (P?=?0.003, P?=?0.004). Frequencies of genotypes TT of rs2296188 and rs2296283 were declined in patients compared with healthy controls (P?=?0.039, P?=?0.033). CC genotype of rs7324510 and rs9582036 was negatively correlated with SLE risk (OR?=?0.538, OR?=?0.508). Distribution of GG, GC, GG?+?GC genotypes of rs9554322 were different between SLE patients and healthy controls (P?=?0.027, P?=?0.036, P?=?0.010). Moreover, frequency of TC genotype of rs7324510 was higher in SLE patients with lupus headache (?2?=?9.924, P?=?0.039) and frequency of TC genotype of rs9943922 was lower in patients with cylindruriain (?2?=?7.589, P?=?0.026). Frequencies of allele C of rs7324510 and allele T of rs9943922 were decreased in SLE patients with cylindruria and hypocomplementemia, respectively (?2?=?4.195, P?=?0.041, ?2?=?3.971, P?=?0.046). However, frequency of allele C of rs9554322 was increased in SLE patients with pyuria (?2?=?11.702, P?=?0.001). In addition, SLE patients carrying GG, GC, CC genotypes for rs9554322 had higher levels of serum sVEGFR-1. In conclusion, serum sVEGFR-1 was elevated in SLE patients and may be a disease marker. VEGFR1 gene polymorphisms related to risk of SLE in a Chinese Han population.

Project description:BackgroundA number of observational studies have been conducted to investigate the association of the IL-10 gene polymorphisms with systemic lupus erythematosus (SLE) susceptibility. However, their results are conflicting.MethodWe searched published case-control studies on the IL-10 polymorphisms and SLE in PubMed, EMBASE and Chinese Biomedical Literature Database. A meta-analysis was conducted using a fixed-effect or random-effect model based on between-study heterogeneity.ResultsA total of 42 studies with 7948 cases and 11866 controls were included in this meta-analysis. Among Caucasians, the CA27 allele of the IL10.G microsatellites (OR 2.38, 95% CI 1.01-5.62), the G allele of the IL-10 -1082G/A polymorphism (G vs. A: OR 1.21, 95% CI 1.02-1.44; GG vs. AA: OR 1.45, 95% CI 1.16-1.82; GG+GA vs. AA: OR 1.16, 95% CI 1.03-1.29) and its associated haplotype -1082G/-819C/-592C (OR 1.25, 95% CI 1.10-1.42) were associated with increased SLE susceptibility without or with unimportant between-study heterogeneity. Removing studies deviating from Hardy-Weinberg equilibrium (HWE) hardly changed these results. Among Asians, the CA21 allele of the IL-10.G microsatellites (OR 1.28, 95% CI 1.02-1.60) and the -1082G/-819C/-592C haplotype (OR 1.24, 95% CI 1.00-1.53) were associated with increased SLE susceptibility, but with substantial between-study heterogeneity or sensitive to HWE status. Removing studies deviating from HWE also produced statistically significant associations of the IL-10 -1082G/A (GG vs. AA: OR 3.21, 95% CI 1.24-8.28; GG vs. AA+GA: OR 2.85, 95% CI 1.19-6.79) and -592C/A polymorphisms (CC+CA vs. AA: OR 0.69, 95% CI 0.51-0.94) with SLE among Asians.ConclusionThis meta-analysis showed that the IL10.G microsatellites, the IL-10 -1082G/A and -592C/A polymorphisms and the haplotype -1082G/-819C/-592C are associated with SLE susceptibility. Besides, this is the first time to report an association between the CA27 allele of the IL-10.G microsatellites and SLE among Caucasians. Further studies are needed to confirm these findings.

Dataset Information

Apolipoprotein E Gene Polymorphisms in Saudi Patients with Systemic Lupus Erythematosus.

Publications

Apolipoprotein E Gene Polymorphisms in Saudi Patients with Systemic Lupus Erythematosus.

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