Expression of Wnt/?-Catenin Signaling Pathway and Its Regulatory Role in Type I Collagen with TGF-?1 in Scleral Fibroblasts from an Experimentally Induced Myopia Guinea Pig Model.

ABSTRACT: Background. To investigate Wnt/?-catenin signaling pathway expression and its regulation of type I collagen by TGF-?1 in scleral fibroblasts from form-deprivation myopia (FDM) guinea pig model. Methods. Wnt isoforms were examined using genome microarrays. Scleral fibroblasts from FDM group and self-control (SC) group were cultured. Wnt isoforms, ?-catenin, TGF-?1, and type I collagen expression levels were examined in the two groups with or without DKK-1 or TGF-?1 neutralizing antibody. Results. For genome microarrays, the expression of Wnt3 in FDM group was significantly greater as confirmed in retinal and scleral tissue. The expression of Wnt3 and ?-catenin significantly increased in FDM group and decreased significantly with DKK-1. TGF-?1 expression level decreased significantly in FDM group and increased significantly with DKK-1. Along with morphological misalignment inside and outside cells, the amount of type I collagen decreased in FDM group. Furthermore, type I collagen increased and became regular in DKK-1 intervention group, whereas it decreased and rearranged more disorder in TGF-?1 neutralizing antibody intervention group. Conclusions. The activation of Wnt3/?-catenin signaling pathway was demonstrated in primary scleral fibroblasts in FDM. This pathway further reduced the expression of type I collagen by TGF-?1, which ultimately played a role in scleral remodeling during myopia development.

SUBMITTER: Li M 

PROVIDER: S-EPMC4877496 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature