Ontology highlight
ABSTRACT:
SUBMITTER: Yoon DS
PROVIDER: S-EPMC4878318 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Yoon Dong Suk DS Pendergrass DeQwon L DL Lee Myon-Hee MH
MethodsX 20160506
Imaging of RNAs and proteins in specific tissues has opened ample avenues to understand gene expression during development. Recently, a fluorescent in situ RNA hybridization (FISH) method has been developed to analyze the spatio-temporal expression patterns of endogenous mRNAs. However, combining FISH with immunofluorescence is challenging as the reaction conditions for the two procedures conflict in multiple ways. In this report, we developed a simple and rapid method to detect both RNAs and as ...[more]