Ontology highlight
ABSTRACT:
SUBMITTER: Bertok T
PROVIDER: S-EPMC4878713 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Bertok Tomas T Gemeiner Pavol P Mikula Milan M Gemeiner Peter P Tkac Jan J
Mikrochimica acta 20121030 1
We report on an ultrasensitive label-free lectin-based impedimetric biosensor for the determination of the sialylated glycoproteins fetuin and asialofetuin. A sialic acid binding agglutinin from <i>Sambucus nigra</i> I was covalently immobilised on a mixed self-assembled monolayer (SAM) consisting of 11-mercaptoundecanoic acid and 6-mercaptohexanol. Poly(vinyl alcohol) was used as a blocking agent. The sensor layer was characterised by atomic force microscopy, electrochemical impedance spectrosc ...[more]