Ontology highlight
ABSTRACT:
SUBMITTER: Gottschalk LB
PROVIDER: S-EPMC4879073 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Gottschalk Laura B LB Vecchio-Pagan Briana B Sharma Neeraj N Han Sangwoo T ST Franca Arianna A Wohler Elizabeth S ES Batista Denise A S DA Goff Loyal A LA Cutting Garry R GR
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society 20151213 3
<h4>Background</h4>Analysis of the functional consequences and treatment response of rare CFTR variants is challenging due to the limited availability of primary airways cells.<h4>Methods</h4>A Flp recombination target (FRT) site for stable expression of CFTR was incorporated into an immortalized CF bronchial epithelial cell line (CFBE41o-). CFTR cDNA was integrated into the FRT site. Expression was evaluated by western blotting and confocal microscopy and function measured by short circuit curr ...[more]