Dataset Information

Prevalence and Fate of Carbapenemase Genes in a Wastewater Treatment Plant in Northern China.

ABSTRACT: Carbapenemase-producing strains of bacteria, which were primarily found in the medical field, have increasingly been found in the environment, thus posing potential risks to public health. One possible way for carbapenemase genes to enter the environment is via wastewater. Therefore, the goal of this study was to determine the occurrence and fate of five high-risk carbapenemase genes in a wastewater treatment plant (WWTP) in northern China using real-time qPCR. Results showed that the blaKPC-2, blaGES-1, and blaIMP-1 genes prevailed throughout all processing stages (even in the chlorination disinfection unit) in the WWTP, whereas the blaVIM-2 and blaOXA-48 genes were not detected in all samples. Worryingly, considerable amounts of carbapenemase genes ((1.54 ± 0.61) × 103 copies/mL to (2.14± 0.41) × 105 copies/mL) were detected in WWTP effluent samples, while the majority of the carbapenemase genes were transported to the dewatered sludge with concentrations from (6.51 ± 0.14) × 109 copies/g to (6.18 ± 0.63) × 1010 copies/g dry weight. Furthermore, a total of 97 KPC-2-producing strains, belonging to 8 bacterial genera, were isolated from the WWTP. Sequencing of 16S rRNA revealed that most of KPC-2 producing isolates were opportunistic pathogens, including Klebsiella spp. (10.3%), Enterococcus spp. (11.3%), Acinetobacter spp. (19.6%), Escherichia spp. (12.4%), Shigella spp. (17.5%), Stenotrophomonas spp. (10.3%) and Wautersiella spp. (9.3%). Moreover, blaKPC-2 genes were identified for the first time in Paenibacillus spp. isolates (an indigenous bacteria), indicating an increased risk of horizontal transfer between clinical pathogens and environmental bacteria. Indeed, a conjugation experiment demonstrated transfer of the blaKPC-2 gene to an E.coli J53 strain from a Klebsiella strain isolated from the WWTP. To our knowledge, this is the first study to obtain Paenibacillus spp. isolates carrying the carbapenemase gene and to quantify the abundance of carbapenemase genes in the environment.

SUBMITTER: Yang F

PROVIDER: S-EPMC4882038 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Prevalence and Fate of Carbapenemase Genes in a Wastewater Treatment Plant in Northern China.

Yang Fengxia F Mao Daqing D Zhou Hao H Luo Yi Y

PloS one 20160526 5

Carbapenemase-producing strains of bacteria, which were primarily found in the medical field, have increasingly been found in the environment, thus posing potential risks to public health. One possible way for carbapenemase genes to enter the environment is via wastewater. Therefore, the goal of this study was to determine the occurrence and fate of five high-risk carbapenemase genes in a wastewater treatment plant (WWTP) in northern China using real-time qPCR. Results showed that the blaKPC-2, ...[more]

PMID: 27227329

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Project description:Carbapenemase-producing bacteria (CPB) are rare, multidrug resistant organisms most commonly associated with hospitalized patients. Metropolitan wastewater treatment plants (WWTP) treat wastewater from large geographic areas which include hospitals and may serve as epidemiologic reservoirs for the maintenance or expansion of CPB that originate from hospitals and are ultimately discharged in treated effluent. However, little is known about the potential impact of these WWTP CPB on the local surface water and their risk to the public health. In addition, CPB that are present in surface water may ultimately disseminate to intensively-managed animal agriculture facilities where there is potential for amplification by extended-spectrum cephalosporins. To better understand the role of WWTPs in the dissemination of CPB in surface waters, we obtained samples of treated effluent, and both upstream and downstream nearby surface water from 50 WWTPs throughout the US. A total of 30 CPB with clinically-relevant genotypes were recovered from 15 WWTPs (30%) of which 13 (50%) serviced large metropolitan areas and 2 (8.3%) represented small rural populations (P < 0.05). Recovery of CPB was lowest among WWTPs that utilized ultraviolet radiation for primary disinfection (12%), and higher (P = 0.11) for WWTPs that used chlorination (42%) or that did not utilize disinfection (50%). We did not detect a difference in CPB recovery by sampling site, although fewer CPB were detected in upstream (8%) compared to effluent (20%) and downstream (18%) samples. Our results indicate that WWTP effluent and nearby surface waters in the US are routinely contaminated with CPB with clinically important genotypes including those producing Klebsiella pneumoniae carbapenemase (KPC) and New Delhi metallo-beta-lactamase (NDM). This is a concern for both public health and animal agriculture because introduction of CPB into intensively managed livestock populations could lead to their amplification and foodborne dissemination.

Project description:This comprehensive study addressed the occurrence, seasonal changes, removal efficiencies, and environmental risk assessments of three macrolide antibiotics in five wastewater treatment plants (WWTPs) with conventional and different additional treatment processes. A 1-year monitoring study was conducted, and influents and effluents were collected from Guangzhou (GZ), Shenzhen (SZ), Tai Po (TP), Shatin (ST), and Stonecutters Island (SI) WWTPs. Solid phase extraction and HPLC-MS/MS were used for the pretreatment and determination. The detection limits for azithromycin (AZI), erythromycin (ERY), and roxithromycin (ROX) ranged from 0.80 to 2.13 ng/L for the influent and effluent water samples. AZI was the most abundant antibiotic found in the influents, with average concentrations ranging from 571 ng/L to 1046 ng/L at all the target WWTPs. The seasonal average AZI concentration was the highest in all five WWTPs with the concentration of 984 ng/L in autumn, 849 ng/L in winter, 741 ng/L in summer, and 533 ng/L in spring. The seasonal AZI removal rates in the WWTPs were similar, with an average removal rate above 63.3% from spring to winter. All the treatments in the five WWTPs showed removal abilities for AZI, ERY, and ROX, regardless of the three phase treatments, namely, the UV disinfection process and conventional or chemically enhanced process within the WWTPs. For ERY and ROX, the average total removal rates were significantly decreased in the spring among all five WWTPs, at 53.1% and 57.8%, respectively. The GZ and SZ WWTPs displayed better removal rates than the TP, ST, and SI WWTPs, because the activity underlying the modified A2/O process in the GZ and SZ WWTPs has important effects on the antibiotic removal because the bacteria could produce compact granules and make the antibiotics settle faster in the wastewater. The additional UV disinfection in the SZ WWTP improved the removal efficiencies of the target antibiotics; it enhanced the biodegradability of residual organic pollutants in the WWTP effluent. Moreover, the corresponding environmental risks have been assessed and are viewed as a necessary component of future research.Supplementary informationThe online version contains supplementary material available at 10.1007/s11270-021-05053-y.

Dataset Information

Prevalence and Fate of Carbapenemase Genes in a Wastewater Treatment Plant in Northern China.

Publications

Prevalence and Fate of Carbapenemase Genes in a Wastewater Treatment Plant in Northern China.

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