Intracellular delivery of messenger RNA by recombinant PP7 virus-like particles carrying low molecular weight protamine.

ABSTRACT:

Background

Cell-penetrating peptides (CPPs) have been widely used as carriers to transport different molecules into living cells, whereas messenger RNAs (mRNAs) have been utilized as target molecules for the prevention and treatment of various diseases. However, the instability of CPPs and mRNAs has limited their application. Bacteriophage PP7 virus-like particles (VLPs) may protect peptides and RNAs from degradation through displaying foreign peptides on their surface and encapsidating RNA linked with the pac site.

Results

In this study, the cDNA of the PP7 coat protein single-chain dimer carrying low molecular weight protamine (LMWP) and the cDNA of green fluorescent protein (GFP) were inserted into two multiple cloning sites of pETDuet-1, respectively. PP7 VLPs carrying t

SUBMITTER: Sun Y 

PROVIDER: S-EPMC4884372 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature