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Lanthanum induced B-to-Z transition in self-assembled Y-shaped branched DNA structure.

ABSTRACT: Controlled conversion of right-handed B-DNA to left-handed Z-DNA is one of the greatest conformational transitions in biology. Recently, the B-Z transition has been explored from nanotechnological points of view and used as the driving machinery of many nanomechanical devices. Using a combination of CD spectroscopy, fluorescence spectroscopy, and PAGE, we demonstrate that low concentration of lanthanum chloride can mediate B-to-Z transition in self-assembled Y-shaped branched DNA (bDNA) structure. The transition is sensitive to the sequence and structure of the bDNA. Thermal melting and competitive dye binding experiments suggest that La(3+) ions are loaded to the major and minor grooves of DNA and stabilize the Z-conformation. Our studies also show that EDTA and EtBr play an active role in reversing the transition from Z-to-B DNA.

SUBMITTER: Nayak AK

PROVIDER: S-EPMC4886512 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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Lanthanum induced B-to-Z transition in self-assembled Y-shaped branched DNA structure.

Nayak Ashok K AK Mishra Aseem A Jena Bhabani S BS Mishra Barada K BK Subudhi Umakanta U

Scientific reports 20160531

Controlled conversion of right-handed B-DNA to left-handed Z-DNA is one of the greatest conformational transitions in biology. Recently, the B-Z transition has been explored from nanotechnological points of view and used as the driving machinery of many nanomechanical devices. Using a combination of CD spectroscopy, fluorescence spectroscopy, and PAGE, we demonstrate that low concentration of lanthanum chloride can mediate B-to-Z transition in self-assembled Y-shaped branched DNA (bDNA) structur ...[more]

PMID: 27241949

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