Ontology highlight
ABSTRACT:
SUBMITTER: Legant WR
PROVIDER: S-EPMC4889433 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Legant Wesley R WR Shao Lin L Grimm Jonathan B JB Brown Timothy A TA Milkie Daniel E DE Avants Brian B BB Lavis Luke D LD Betzig Eric E
Nature methods 20160307 4
Extending three-dimensional (3D) single-molecule localization microscopy away from the coverslip and into thicker specimens will greatly broaden its biological utility. However, because of the limitations of both conventional imaging modalities and conventional labeling techniques, it is a challenge to localize molecules in three dimensions with high precision in such samples while simultaneously achieving the labeling densities required for high resolution of densely crowded structures. Here we ...[more]