Dissecting the role of transforming growth factor-?1 in topmouth culter immunobiological activity: a fundamental functional analysis.

ABSTRACT: Transforming growth factor-?1 (TGF-?1) has been proven to function primarily in mammalian immunobiological activity, but information regarding the immune role of TGF-?1 in teleosts is limited. In the present study, we describe the cDNA cloning and characterization of the TGF-?1 molecule in the topmouth culter. TGF-?1 is highly expressed in immune-related tissues of the culter, including the thymus, head kidney, and spleen. The recombinant culter TGF-?1 (cTGF-?1) was successfully expressed and purified in vitro, and the effects of cTGF-?1 on the mRNA expression of pro-inflammatory cytokines, such as TNF-? and IL-1?, in the absence or presence of LPS was determined in culter peripheral blood leukocytes. cTGF-?1 was found to have bipolar properties in inflammatory reactions. Additionally, to assess the immune role of teleost TGF-?1 in vivo, the expression of TGF-?1 in the culter thymus and spleen tissues induced by poly I:C were also examined. The expression of TGF-?1 was obviously up-regulated, as shown in the cell lines. However, the peak time of cTGF-?1 expression in the cell lines occurred significantly earlier than in the organic tissues under the same inducer, suggesting that the response of the teleost TGF-?1 molecule to exogenous infection depends on a more complicated signalling pathway in vivo than in vitro.

SUBMITTER: Qi P 

PROVIDER: S-EPMC4890032 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature