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Viable offspring obtained from Prm1-deficient sperm in mice.


ABSTRACT: Protamines are expressed in the spermatid nucleus and allow denser packaging of DNA compared with histones. Disruption of the coding sequence of one allele of either protamine 1 (Prm1) or Prm2 results in failure to produce offspring, although sperm with disrupted Prm1 or Prm2 alleles are produced. Here, we produced Prm1-deficient female chimeric mice carrying Prm1-deficient oocytes. These mice successfully produced Prm1(+/-) male mice. Healthy Prm1(+/-) offspring were then produced by transferring blastocysts obtained via in vitro fertilization using zona-free oocytes and sperm from Prm1(+/-) mice. This result suggests that sperm lacking Prm1 can generate offspring despite being abnormally shaped and having destabilised DNA, decondensed chromatin and a reduction in mitochondrial membrane potential. Nevertheless, these mice showed little derangement of expression profiles.

SUBMITTER: Takeda N 

PROVIDER: S-EPMC4890041 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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Viable offspring obtained from Prm1-deficient sperm in mice.

Takeda Naoki N   Yoshinaga Kazuya K   Furushima Kenryo K   Takamune Kazufumi K   Li Zhenghua Z   Abe Shin-Ichi S   Aizawa Shin-Ichi S   Yamamura Ken-Ichi K  

Scientific reports 20160602


Protamines are expressed in the spermatid nucleus and allow denser packaging of DNA compared with histones. Disruption of the coding sequence of one allele of either protamine 1 (Prm1) or Prm2 results in failure to produce offspring, although sperm with disrupted Prm1 or Prm2 alleles are produced. Here, we produced Prm1-deficient female chimeric mice carrying Prm1-deficient oocytes. These mice successfully produced Prm1(+/-) male mice. Healthy Prm1(+/-) offspring were then produced by transferri  ...[more]

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