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Efficient pDNA Delivery Using Cationic 2-Hydroxypropyl-?-Cyclodextrin Pluronic-Based Polyrotaxanes.

ABSTRACT: A family of cationic Pluronic-based polyrotaxanes (PR(+)), threaded with 2-hydroxypropyl-?-cyclodextrin (HPCD), was synthesized for pDNA delivery into multiple cell lines. All PR(+) formed highly stable, positively charged pDNA complexes that were < 250 nm in diameter. The cellular uptake and pDNA transfection efficiencies of the PR(+):pDNA complexes was enhanced relative to the commercial transfection standards L2K and bPEI, while displaying similar or lower toxicity profiles. Charge density and threading efficiency of the PR(+) agent significantly influenced the colloidal stability and physical properties of the complexes, which impacted their intracellular transfection efficiencies. Taken together, our results suggest that HPCD: Pluronic PR(+) can be used as potent vectors for pDNA-based therapeutics.

SUBMITTER: Badwaik V

PROVIDER: S-EPMC4891183 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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Efficient pDNA Delivery Using Cationic 2-Hydroxypropyl-β-Cyclodextrin Pluronic-Based Polyrotaxanes.

Badwaik Vivek V Mondjinou Yawo Y Kulkarni Aditya A Liu Linjia L Demoret Asher A Thompson David H DH

Macromolecular bioscience 20150810 1

A family of cationic Pluronic-based polyrotaxanes (PR(+)), threaded with 2-hydroxypropyl-β-cyclodextrin (HPCD), was synthesized for pDNA delivery into multiple cell lines. All PR(+) formed highly stable, positively charged pDNA complexes that were < 250 nm in diameter. The cellular uptake and pDNA transfection efficiencies of the PR(+):pDNA complexes was enhanced relative to the commercial transfection standards L2K and bPEI, while displaying similar or lower toxicity profiles. Charge density an ...[more]

PMID: 26257319

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