Dataset Information

The role of transketolase and octulose in the resurrection plant Craterostigma plantagineum.

ABSTRACT: Phylogenetic analysis revealed that Craterostigma plantagineum has two transketolase genes (transketolase 7 and 10) which are separated from the other transketolase genes including transketolase 3 from C. plantagineum We obtained recombinant transketolase 3, 7, and 10 of C. plantagineum and showed that transketolase 7 and 10 of C. plantagineum, but not transketolase 3, catalyse the formation of octulose-8-phosphate in vitro Transketolase 7 and 10 of C. plantagineum performed the exchange reaction that produces octulose-8-phosphate using glucose-6-phosphate and fructose-6-phosphate as substrates. Octulose is localized in the cytosol and phloem exudate analysis showed that octulose was the dominant sugar exported from the leaves to the roots.

SUBMITTER: Zhang Q

PROVIDER: S-EPMC4892735 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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The role of transketolase and octulose in the resurrection plant Craterostigma plantagineum.

Zhang Qingwei Q Linnemann Thomas Vitus TV Schreiber Lukas L Bartels Dorothea D

Journal of experimental botany 20160429 11

Phylogenetic analysis revealed that Craterostigma plantagineum has two transketolase genes (transketolase 7 and 10) which are separated from the other transketolase genes including transketolase 3 from C. plantagineum We obtained recombinant transketolase 3, 7, and 10 of C. plantagineum and showed that transketolase 7 and 10 of C. plantagineum, but not transketolase 3, catalyse the formation of octulose-8-phosphate in vitro Transketolase 7 and 10 of C. plantagineum performed the exchange reactio ...[more]

PMID: 27129952

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Project description:The resurrection plant Craterostigma plantagineum possesses an extraordinary capacity to survive long-term desiccation. To enhance our understanding of this phenomenon, complementary transcriptome, soluble proteome and primary metabolome analyses were carried out on plant leaves collected from different physiological stages during a dehydration and rehydration cycle. A total of 7348 contigs, 611 proteins and 46 metabolites were differentially regulated across stages. Dynamic changes in transcript, protein and metabolite levels revealed a unique signature characterizing each stage. An overall low correlation between transcript and protein abundance suggests a prominent role for post-transcriptional modification in metabolic reprogramming to prepare plants to desiccation and recovery upon rehydration. The integrative analysis of three -omics datasets was performed with an emphasis on photosynthesis, photorespiration, energy metabolism and amino acid metabolism. The results revealed a set of fine regulations/changes that modulate primary metabolism to confer plasticity to metabolic pathways, thus optimizing plant performance under stress. For example, maintenance of cyclic electron flow and photorespiration and switch from C3 to Crassulacean acid metabolism (CAM) photosynthesis may contribute to partially sustain photosynthesis and minimize oxidative damage during dehydration. transcripts with a delayed translation, ATP-independent bypasses, alternative respiratory pathway and 4-aminobutyric acid (GABA) shunt may play a role in energy management, altogether conferring bioenergetic advantages to meet energy demands for survival during desiccation. This knowledge provides a high-resolution map of the changes occurring in the primary metabolism during dehydration and rehydration and enriches the current understanding of the molecular mechanisms underpinning plant desiccation tolerance. The datasets here provided will ultimately inspire biotechnological strategies for drought tolerance improvement in crops.

Dataset Information

The role of transketolase and octulose in the resurrection plant Craterostigma plantagineum.

Publications

The role of transketolase and octulose in the resurrection plant Craterostigma plantagineum.

Similar Datasets

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