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ABSTRACT:
SUBMITTER: DeVore MS
PROVIDER: S-EPMC4894822 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
DeVore Matthew S MS Braimah Adebayo A Benson David R DR Johnson Carey K CK
The journal of physical chemistry. B 20160504 19
We investigate the roles of measurement time scale and the nature of the fluorophores in the FRET states measured for calmodulin, a calcium signaling protein known to undergo pronounced conformational changes. The measured FRET distributions depend markedly on the measurement time scale (nanosecond or microsecond). Comparison of FRET distributions measured by donor fluorescence decay with FRET distributions recovered from single-molecule burst measurements binned over time scales of 90 μs to 1 m ...[more]