Unknown

Dataset Information

0

A recombinant chimeric protein containing B chains of ricin and abrin is an effective vaccine candidate.


ABSTRACT: Both ricin toxin (RT) and abrin toxin (AT) are 2 important toxin agents as potantial bioweapons. A dual subunit vaccine against RT and AT exposure is a promising option for developing prophylactic vaccination. In this study, we constructed a dual vaccine with RT B chain and AT B chain named RTB-ATB. The RTB-ATB chimeric protein was expressed in Escherichia coli (E. coli), and the purified protein was used to evaluate the immune response by a 2 × 2 × 2 × 2 factorial design. The main effects included dose of RTB-ATB, route of immunization injection, immunization time interval, and dose of native toxins challenge. For 2 × LD(50) challenge of RT or AT, 100% of the RTB-ATB immunized mice survived and regained or exceeded their initial weights within 10 days. For 4 × LD(50) challenge, different routes of immunization injection caused significant difference (P < 0.05), intraperitoneal (i.p.) administration of immunogen protected mice better than the subcutaneous (s.c.) administration. In conclusion, when administered i.p. to mice with 25 ?g per mouse and immunization time interval ? in the absence of adjuvant, the chimeric protein elicited a stronger immune response and protected the animals from a dose of native toxins which was 4 times higher than their LD(50) in unvaccinated mice. Besides, the RTB-ATB chimeric protein could induce specific neutralizing antibodies against these 2 toxins. We anticipate that this study will open new possibilities in the preparation of RTB-ATB dual subunit vaccine against the exposure to deadly RT and AT.

SUBMITTER: Wang J 

PROVIDER: S-EPMC4896596 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

altmetric image

Publications

A recombinant chimeric protein containing B chains of ricin and abrin is an effective vaccine candidate.

Wang Junhong J   Gao Shan S   Zhang Tao T   Kang Lin L   Cao Wuchun W   Xu Na N   Liu Wensen W   Wang Jinglin J  

Human vaccines & immunotherapeutics 20140207 4


Both ricin toxin (RT) and abrin toxin (AT) are 2 important toxin agents as potantial bioweapons. A dual subunit vaccine against RT and AT exposure is a promising option for developing prophylactic vaccination. In this study, we constructed a dual vaccine with RT B chain and AT B chain named RTB-ATB. The RTB-ATB chimeric protein was expressed in Escherichia coli (E. coli), and the purified protein was used to evaluate the immune response by a 2 × 2 × 2 × 2 factorial design. The main effects inclu  ...[more]

Similar Datasets

| S-EPMC3169317 | biostudies-literature
| S-EPMC8004863 | biostudies-literature
| S-EPMC3895562 | biostudies-literature
| S-EPMC6468687 | biostudies-literature
| S-EPMC1413738 | biostudies-literature
| S-EPMC10062050 | biostudies-literature
| S-EPMC3536361 | biostudies-literature
| S-EPMC7232224 | biostudies-literature
| S-EPMC7426426 | biostudies-literature
| S-EPMC4708040 | biostudies-literature