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Self-Assembled DNA Immunonanoflowers as Multivalent CpG Nanoagents.

ABSTRACT: Synthetic unmethylated cytosine-guanine (CpG) oligodeoxynucleotides are immunostimulatory motifs that have shown promise as vaccines or adjuvants for diseases such as cancers and infectious diseases. In the present work, novel immuno-nanoflowers (NFs), self-assembled from long DNA integrated with tandem CpG through rolling circle replication, were developed for efficient CpG delivery and protection from nuclease degradation. In a model of macrophage-like cells, the CpG NFs proved to be potent immunostimulators by triggering the proliferation of these immune cells, which, in turn, secreted immunostimulatory cytokines, including tumor necrosis factor ?, interleukin-6, and interleukin-10. These results demonstrate the ability of CpG NFs to induce cancer cell apoptosis and necrosis.

SUBMITTER: Zhang L

PROVIDER: S-EPMC4898273 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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Self-Assembled DNA Immunonanoflowers as Multivalent CpG Nanoagents.

Zhang Liqin L Zhu Guizhi G Mei Lei L Wu Cuichen C Qiu Liping L Cui Cheng C Liu Yuan Y Teng I-Ting IT Tan Weihong W

ACS applied materials & interfaces 20151022 43

Synthetic unmethylated cytosine-guanine (CpG) oligodeoxynucleotides are immunostimulatory motifs that have shown promise as vaccines or adjuvants for diseases such as cancers and infectious diseases. In the present work, novel immuno-nanoflowers (NFs), self-assembled from long DNA integrated with tandem CpG through rolling circle replication, were developed for efficient CpG delivery and protection from nuclease degradation. In a model of macrophage-like cells, the CpG NFs proved to be potent im ...[more]

PMID: 26440045

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