Super-Resolved Traction Force Microscopy (STFM).
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ABSTRACT:
SUBMITTER: Colin-York H
PROVIDER: S-EPMC4898862 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Publications
Super-Resolved Traction Force Microscopy (STFM).
Colin-York Huw H Shrestha Dilip D Felce James H JH Waithe Dominic D Moeendarbary Emad E Davis Simon J SJ Eggeling Christian C Fritzsche Marco M
Nano letters 20160304 4
Measuring small forces is a major challenge in cell biology. Here we improve the spatial resolution and accuracy of force reconstruction of the well-established technique of traction force microscopy (TFM) using STED microscopy. The increased spatial resolution of STED-TFM (STFM) allows a greater than 5-fold higher sampling of the forces generated by the cell than conventional TFM, accessing the nano instead of the micron scale. This improvement is highlighted by computer simulations and an acti ...[more]
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