TGF? signaling promotes matrix assembly during mechanosensitive embryonic salivary gland restoration.

ABSTRACT: Mechanical properties of the microenvironment regulate cell morphology and differentiation within complex organs. However, methods to restore morphogenesis and differentiation in organs in which compliance is suboptimal are poorly understood. We used mechanosensitive mouse salivary gland organ explants grown at different compliance levels together with deoxycholate extraction and immunocytochemistry of the intact, assembled matrices to examine the compliance-dependent assembly and distribution of the extracellular matrix and basement membrane in explants grown at permissive or non-permissive compliance. Extracellular matrix and basement membrane assembly were disrupted in the glands grown at low compliance compared to those grown at high compliance, correlating with defective morphogenesis and decreased myoepithelial cell differentiation. Extracellular matrix and basement membrane assembly as well as myoepithelial differentiation were restored by addition of TGF?1 and by mechanical rescue, and mechanical rescue was prevented by inhibition of TGF? signaling during the rescue. We detected a basal accumulation of active integrin ?1 in the differentiating myoepithelial cells that formed a continuous peripheral localization around the proacini and in clefts within active sites of morphogenesis in explants that were grown at high compliance. The pattern and levels of integrin ?1 activation together with myoepithelial differentiation were interrupted in explants grown at low compliance but were restored upon mechanical rescue or with application of exogenous TGF?1. These data suggest that therapeutic application of TGF?1 to tissues disrupted by mechanical signaling should be examined as a method to promote organ remodeling and regeneration.

SUBMITTER: Peters SB 

PROVIDER: S-EPMC4899049 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature