Ontology highlight
ABSTRACT:
SUBMITTER: Shema E
PROVIDER: S-EPMC4904710 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Shema Efrat E Jones Daniel D Shoresh Noam N Donohue Laura L Ram Oren O Bernstein Bradley E BE
Science (New York, N.Y.) 20160501 6286
Different combinations of histone modifications have been proposed to signal distinct gene regulatory functions, but this area is poorly addressed by existing technologies. We applied high-throughput single-molecule imaging to decode combinatorial modifications on millions of individual nucleosomes from pluripotent stem cells and lineage-committed cells. We identified definitively bivalent nucleosomes with concomitant repressive and activating marks, as well as other combinatorial modification s ...[more]