Ontology highlight
ABSTRACT:
SUBMITTER: Thyme SB
PROVIDER: S-EPMC4906408 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Thyme Summer B SB Akhmetova Laila L Montague Tessa G TG Valen Eivind E Schier Alexander F AF
Nature communications 20160610
The CRISPR/Cas system uses guide RNAs (gRNAs) to direct sequence-specific DNA cleavage. Not every gRNA elicits cleavage and the mechanisms that govern gRNA activity have not been resolved. Low activity could result from either failure to form a functional Cas9-gRNA complex or inability to recognize targets in vivo. Here we show that both phenomena influence Cas9 activity by comparing mutagenesis rates in zebrafish embryos with in vitro cleavage assays. In vivo, our results suggest that genomic f ...[more]