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A Farnesyltransferase Acts to Inhibit Ectopic Neurite Formation in C. elegans.

ABSTRACT: Genetic pathways that regulate nascent neurite formation play a critical role in neuronal morphogenesis. The core planar cell polarity components VANG-1/Van Gogh and PRKL-1/Prickle are involved in blocking inappropriate neurite formation in a subset of motor neurons in C. elegans. A genetic screen for mutants that display supernumerary neurites was performed to identify additional factors involved in this process. This screen identified mutations in fntb-1, the ? subunit of farnesyltransferase. We show that fntb-1 is expressed in neurons and acts cell-autonomously to regulate neurite formation. Prickle proteins are known to be post-translationally modified by farnesylation at their C-terminal CAAX motifs. We show that PRKL-1 can be recruited to the plasma membrane in both a CAAX-dependent and CAAX-independent manner but that PRKL-1 can only inhibit neurite formation in a CAAX-dependent manner.

SUBMITTER: Carr D

PROVIDER: S-EPMC4907426 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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A Farnesyltransferase Acts to Inhibit Ectopic Neurite Formation in C. elegans.

Carr David D Sanchez-Alvarez Leticia L Imai Janice H JH Slatculescu Cristina C Noblett Nathaniel N Mao Lei L Beese Lorena L Colavita Antonio A

PloS one 20160614 6

Genetic pathways that regulate nascent neurite formation play a critical role in neuronal morphogenesis. The core planar cell polarity components VANG-1/Van Gogh and PRKL-1/Prickle are involved in blocking inappropriate neurite formation in a subset of motor neurons in C. elegans. A genetic screen for mutants that display supernumerary neurites was performed to identify additional factors involved in this process. This screen identified mutations in fntb-1, the β subunit of farnesyltransferase. ...[more]

PMID: 27300162

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